Figure 6. SIRT1 and SIRT2 localize differentially in the brain.
Immunofluorescence microscopy using specific SIRT1 and SIRT2 antibodies in cortex, hippocampus and striatum. SIRT1 (green) co-localizes with the neuronal marker NeuN (red) and DAPI (4′,6-diamidino-2-phenylindole; blue) in cortical neurons, hippocampal granular neurons and striatal neurons, while SIRT2 does not co-label with NeuN (neuron-specific nuclear-proteins) or DAPI; it mainly co-localizes with the fibres of myelinated axons in white matter and oligodendrocytes. Samples were processed as described previously [141].