Figure 1.
Strontium ranelate increases osteoprotegerin (OPG) mRNA and protein expression in human osteoblasts (HOBs). Cells were exposed for 24 h to vehicle or: 0.01, 0.1, 1 and 2 mM strontium ranelate, before RNA was extracted, converted to cDNA and then analysed by real-time reverse transcription PCR. (A) OPG mRNA expression was quantified and expressed as % of the value after treatment with vehicle (% vehicle). The results are presented as means ± SEM from four experiments. (B) OPG protein was measured by elisa according to manufacturer's instructions. The results are representative of three experiments ± SD each with triplicates. Concentrations are expressed as added to the vehicle, which contains 1 mM Ca2+. ***P < 0.001 compared with vehicle; *P < 0.05 compared with vehicle.