Figure 7.
Tmem27−/y animals exhibit decreased plasma levels of a number of short-chain acylcarnitine species with corresponding urinary losses of a subset of those species. A, Plasma FC and acylcarnitine levels from animals fasted overnight were measured by mass spectrometry. n = 13–14. *, P < 0.0001 (FC, C2, C3), P = 0.0011 (C4), and P = 0.019 (C4-DC) vs. Tmem27+/y. B, The 24-h urine excretion of FC and acylcarnitines normalized to creatinine. Animals were housed individually with free access to food and water in metabolic cages for 24-h urine collection. FC, acylcarnitines, and creatinine were quantified by mass spectrometry. n = 11–12. *, P ≤ 0.05 vs. Tmem27+/y. C, Fractional excretions (FE) of FC and acylcarnitines were calculated using acylcarnitine and creatinine concentrations obtained from spot plasma and urine samples collected after an overnight fast. FE (percent) = ([AC]urine × [Cr]plasma)/([AC]plasma × [Cr]urine). C3 and C5:1: n = 5–8. *, P ≤ 0.05 vs. Tmem27+/y. C4-DC: n = 3–5. †, P = 0.07 vs. Tmem27+/y. Data represent the mean ± sem.