Figure 6.
There is no difference in isolated islet function on NC and HFD at 6 months of age. A, Islets were isolated from Tmem27+/y and Tmem27−/y animals by Liberase R1 enzyme digestion, maintained in culture, and stimulated with 5.5 and 16.7 mm glucose. Secreted insulin was determined by RIA and normalized to total cellular protein content. Left, Control diet; right, HFD. B, Relative increase in insulin secretion with 16.7 mm glucose compared with 5.5 mm glucose on control diet and HFD. Data represent the mean ± sem. of two independent experiments, each of which was performed in triplicates of 20 islets per condition collected from four animals of each genotype.