Fig. 3.
Global gene expression of RacCA tumors resembles KS and KSHV-induced Kaposi-like mouse tumors. (A) Heat map representation of 450 significantly up- or down-regulated genes (P < 0.05) from the 723 human KS signature genes that were present on the Affymetrix mouse 430 2.0 chip. A total of 408 genes (91%) in this 408-gene signature were equally up- or down-regulated between RacCA-mKS and KS sample groups. Control mouse and human SCC samples possess less than 10% of the KS signatures. Selected up- (red) or down-regulated (blue) genes are listed on the right. The full list of genes is available in Table S2. (B) Distribution of KS signature. Dark gray, genes shared by KS, RacCA-mKS, and KSHV-mKS; orange, genes shared by KS and RacCA-mKS; green, genes shared by KS and KSHV-mKS; yellow, genes in KS only. Gene list is available in Table S3. (C) Up-regulation of angiogenic gene expression in RacCA tumors compared with surrounding uninvolved tissue. Bars represent mean fold increase (triplicates ± SEM) in mRNA levels quantified by real-time quantitative RT-PCR.