Figure 5. Effects of Ca2+, pH and contraction on in vitro eEF2 kinase activity.
Top panel: eukaryotic elongation factor 2 (eEF2) kinase was immunoprecipitated from rat skeletal muscle samples and activity was measured in vitro in the absence and presence of Ca2+ at pH 7.2 or 6.8. Data are mean ±s.e.m. from 3 samples. *Main effect of Ca2+, P < 0.01. Bottom panel: rat gastrocnemius muscle samples before and during in situ contractions were processed and immunoprecipitated eEF2 kinase activity was measured in vitro. AU, arbitrary units. Data are mean ±s.e.m. from 5 samples.