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. Author manuscript; available in PMC: 2009 Nov 1.
Published in final edited form as: Nat Methods. 2009 Mar 31;6(5):363–369. doi: 10.1038/nmeth.1323

Figure 4. Characterization of integration-free iPSCs.

Figure 4

(a) Normal karyotype of the integration-free iPSC line iPS25Δ1. (b) RT-PCR analyses of integration-free iPSC lines, compared to expression in ESCs. (c) Immunofluorescent analysis of Oct4 and Nanog expression in integration-free iPSCs. (d) Bisulphite sequencing of the promoter region of Oct4 and Nanog. Open and closed circles indicate unmethylated and methylated CpG dinucleotides, respectively. (e) Teratomas generated from integration-free iPSCs, showing differentiation to all three germlayers. Scale bar, 100 μm

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