Figure 1.
Ang2 and c-fms are upregulated in the ischemic hindlimb whereas Ang1 is not. C57Bl/6J mice underwent femoral artery ligation. RNA was collected after 2, 5, or 7 days from the ischemic and nonischemic adductor muscle. Real-time PCR was performed for Ang2 (A), Ang1 (B), and c-fms (C). D, Whole mount immunohistochemistry was performed after 2, 5, or 7 days on the nonischemic and ischemic adductor muscle for Ang2 (green) and PECAM-1 (red). Shown are confocal images of the proximal region of the adductor muscle. (mean±SEM, n=4 to 6; *P<0.05; #P<0.05 compared to day 5 and day 7).