FIG. 2.
(A) Tetracycline-induced inhibition of ERβ expression in T47D-ERβ cells measured via the concurrent expression of EGFP after 24 h of exposure at 100% cell density. Fluorescence is expressed relative to maximum expression at 0 ng/ml of tetracycline set at 100%. No fluorescence is observed above 150 ng of tetracycline/ml. Each data point represents the mean of triplicate exposure ± SD. (B) T47D-ERβ tet-off FLAG-ERβ cells were cultured in the presence (1000 and 10 ng/ml) or absence of tetracycline for 24 h, 14C8 antibody was used to detect ERβ in the T47D wild type, and Coomassie staining was used as loading control. Quantification of the intensity of the ERβ-FLAG bands was measured by ImageJ.