Table 1.
Plasmids and oligonucleotides used in this work
| Plasmids | ||
| pFC | Psychrophilic gene-expression vector, containing the T/R box, the promoter and termination region of the P. haloplanktis TAC125 aspC gene and the chloramphenicol resistance gene | [6] |
| pFCamyΔCt | pFC containing a truncated version of the amy gene devoid of the C-terminal propeptide encoding portion | [6] |
| pFCamyΔCt-blaM | pFC containing the amyΔCt-blaM gene encoding the chimerical protein Amy-BlaM | [6] |
| pVS | pGEM7Z vector containing a conjugation transfer origin (oriT), the counter selectable marker pheS Gly294 and the ampicillin resistance gene | This work |
| Oligonucleotides | ||
| Oligo oriTEcoRIfw | 5'-TTGAATTCTCGCACGATATACAGG-3' | |
| Oligo oriTSacIrv | 5'-AAGAGCTCTTGAAGACGAAAGGG-3' | |
| Oligo gspESphIfw | 5'-TTGCATGCATGCGCATCATCCGG-3' | |
| Oligo gspESacIrv | 5'-AAGAGCTCCAATATCGAGCTTAGCC-3' | |
| Oligo gspESacIfw | 5'-TTGAGCTCCTAAAGTAGGTATGACC-3' | |
| Oligo gspEEcoRIrv | 5'-AAGAATTCGTACACGGGCTACAGCC-3' | |
| Oligo gspESrv | 5'-AAGAGCTCCTTCACTGAGCATCG-3' | |
| Oligo gspEfw | 5'-GCAATTTAAGCAGCGCGAAGATG-3' | |
| Oligo gspErv | 5'-ATCTAGGGCACGGTATTCAAATGC-3' | |
| PheSSNfw | 5'-TTGTCGACATATGTCACATCTCGCAGAAC-3' | |
| PheSXrv | 5'-CCTCTAGAGAATTTCATAATCTATTCCTGCC-3' |