Figure 5.
LTαβ–LTβR interactions are required for optimal CD4 priming in vivo. (A) C57BL/6 mice received CFSE-labeled, OVA-specific WT- or LTβ−/−-OTII T cells, were immunized with OVA+LPS, or were left unimmunized. At 36 h after immunization, OTII CFSE content was assessed, comparing baseline CFSE content for each individual genotype. At day 5 after immunization, secretion of IFNγ from OTII T cells was assessed by intracellular FACS. (B) C57BL/6 mice received OVA-specific WT-OTII T cells, were treated with huIgG or LTβR-Ig, and were immunized with OVA+LPS. At day 7, the frequency of IFNγ+ OTII T cells was assessed. The experiments were performed twice with three to five mice per group in each experiment.