Figure 4.
Epithelial barrier dysfunction in SAMP and AKR BM → SAMP mice. (A) TEER assays on ileum and jejunum from SAMP (n = 8), AKR (n = 8), SAMP BM → SAMP (n = 5), AKR BM → SAMP (n = 4), AKR BM → AKR (n = 3), and SAMP BM → AKR (n = 4) mice. Ilea from SAMP, SAMP BM → SAMP, and AKR BM → SAMP mice exhibited decreases in ΔTEER indicative of increased epithelial permeability, whereas ilea from AKR, AKR BM → AKR, and SAMP BM → AKR mice maintained an effective epithelial resistance barrier. Significant differences (P < 0.03) are indicated between experimental groups (A, left). No differences were observed in jejunal TEER among BMT recipient groups. A significant in crease in TEER was seen in native SAMP compared with AKR jejunum; P < 0.03 (A, right). (B) In vivo assays measuring epithelial permeability to solutes in the small intestine and colon using urinary FE ratio of lactulose to mannitol (B, left) and of sucralose (B, right), respectively, in AKR BM → SAMP (♦) (n = 6) and SAMP BM → AKR (▪) (n = 6) mice. The time 0 point was collected immediately before irradiation and transplantation and thus represent the permeability of native SAMP (♦) and AKR (▪) tissues. *Indicates significantly increased (P < 0.05) compared with SAMP BM → AKR mice.