Figure 4.
Mobilization of LX+ neutrophils and B cells from the spleen to the bone marrow leads to robust monocyte labeling. (A) LX beads were injected i.v. Then, 8 h later, some mice received CLLs i.v. (middle dot plot). The blood was analyzed 12 h later, 20 h after the administration of LX beads, and at several time points thereafter. At the 12-h time point, most LX+ cells seen in the group of mice receiving CLLs were CD115− cells with the high FSC/SSC profile of neutrophils (middle and right dot plots). A comparison with mice receiving no liposomes, only LX i.v., is shown in the left dot plot. (B) A time course depicts the fraction of LX+ cells among total bone marrow cells that were CD115− (mostly neutrophils and B cells) and CD115+Gr-1hi monocytes from 4 h to 2 d after the administration of CLL. Error bars represent SEM. (C) Bone marrow was analyzed 16 h after liposome administration. Monocytes were identified by double staining for F4/80 and CD115, and the degree to which this population was LX+ was assessed (right plots). Comparison was made with mice that received only LX beads without CLL (left plots). (D) Analysis of the monocyte population in blood at day 2 or later after CLLs were administered. All data are representative of more than six mice per condition.