Figure 2.
Effects of temperature on NESA. Reactions were set up as detailed using the standard NESA procedure described in Methods using the indicated fluorescent probes and 10 fmol of each complement oligonucleotide. Reactions were then analyzed using capillary electrophoresis. (A) Reactions were incubated for 1 h at the indicated temperatures. (B) Buffer, probe and enzyme were incubated together at the indicated temperatures for 15 min. Reactions were then equilibrated at 58°C, complement added, and incubation continued for 1 h at 58°C.