FIG. 1.
MHV and SARS-CoV induce Cxcl2 mRNA expression but prevent CXCL2 protein synthesis. L cells were infected with MHV, or L-ACE2 cells were infected with SARS-CoV, at an MOI of 10. (A) Release of infectious MHV was determined by a plaque assay, and isolated total RNA was analyzed for Cxcl2 expression by RT-qPCR. Values were normalized to GAPDH and expressed as induction (n-fold). RU, relative units. (B) Supernatants from MHV-infected L cells and SARS-CoV-infected L-ACE2 cells were analyzed for protein production by a CXCL2-specific ELISA. SeV infection was used as a positive control.