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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: J Mol Cell Cardiol. 2007 Apr 27;43(1):73–84. doi: 10.1016/j.yjmcc.2007.04.014

Figure 1. Adiponectin expression in cardiomyocytes.

Figure 1

Figure 1

Figure 1

Figure 1

a. Quantitative real time RT-PCR measurement of adiponectin relative to β-actin in cultured cardiomyocytes isolated from adult rats and in cultured differentiated adipocytes. Data are expressed as mean±SEM, n=6, * p<0.01. b. Quantitative real time RT-PCR measurement of aP2 relative to β-actin with equal loading of cDNA samples from cultured adult rat cardiomyocytes and differentiated adipocytes. Data are expressed as mean±SEM, n=4, * p<0.01. c. Western blot analyses of adiponectin protein in protein samples extracted from cultured adipocytes, adult and neonatal rat cardiomyocytes (ARC and NRC, respectively). Preincubation of adiponectin blocking peptide serves as negative control. The relative levels of adiponectin were normalized to actin. Data are expressed as mean±SEM, n=4, * p<0.01. d. Immunofluorescent staining with anti-adiponectin on cultured cardiomyocytes. The negative controls were stained with IgG only. Experiments were performed three times with similar results.

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