Figure 1.
Verification of correct pSC101BADγβαA[hygro] (Red/ET (hygro) and pSC101BADγβαA-I-SceI[amp] recombinants. (a) Correct pSC101BADγβαA[hygro] recombinants were identified by digestion with NcoI (fragment sizes 4.8, 2.3 and 2.0 kb). (b) Correct pSC101BADγβαA-I-SceI[amp] clones were identified by digestion with SspI (fragment sizes 4.6, 4.1 and 2.0 kb). *Negative clone.