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Expression of Spred-1 and Spred-2 in LECs and BECs. (A) FACS profile of CD45− CD31+ cells from E14.5 embryos. (B) The relative mRNA levels of the indicated genes determined by quantitative real-time PCR. Total RNA (1 μg) isolated from approximately 5.0 × 104 FACS-sorted LECs, BECs, and DPs was analyzed (n = 3). The error bars indicate SEM.
