Fig. 4.
Rapid cell death and IL-1β release induced by the FTT0748 and FTT0584 mutants depends on host caspase-1 and ASC. Wild-type, caspase-1−/− and ASC−/− bone marrow-derived macrophages were activated with heat-killed F. novicida for 12 h and then infected with 10 bacteria per macrophage of the indicated strains. (A) Cell death was quantified by LDH release. (B) IL-1β in the supernatant was quantified by ELISA at 6 h after infection. Data are representative of three independent experiments. ND, not detected.