FIGURE 6.
Effect of platelet or leukocyte MPs from preeclamptics in 5-HT-induced contraction in mice aortic rings. A: Flow cytometry histograms showing the surface expression levels of GPIbα in total PrMP samples and in fractions obtained after separation of MPs as described in Materials and Methods. Fluorescence (FL2, red fluorescence channel intensity, log scale) is expressed in arbitrary units (a.u.). B: Concentration-effect curves to 5-HT in mouse aortic rings (n = 5) after incubation for 24 hours with either platelet MPs (▪) or leukocytes MPs (•) from preeclamptics or medium without MPs (□). C: Concentration-effect curves to 5-HT in the absence (□) and in the presence of the NO synthase inhibitor (○) L-NA (100 μmol/L) or of NS-398 (10 μmol/L) (⋄) or of L-NA plus NS-398 (▵) in mouse aortic rings incubated with platelet MPs from preeclamptics for 24 hours (n = 5). D: Concentration-effect curves to 5-HT in the absence (▪) and in the presence of the NO synthase inhibitor (•) L-NA (100 μmol/L) or of NS-398 (10 μmol/L) (♦) or of L-NA plus NS-398 (▴) in mouse aortic rings incubated with leukocyte MPs from preeclamptics for 24 hours (n = 5). **P < 0.01, ***P < 0.001, significantly different data obtained in the absence of inhibitors.