Figure 4. The nanos1 3′UTR allows protein expression in PGCs in the presence of miRNAs.
(A) Schematic of GFP-nanos1 3′UTR reporter constructs. Black bar indicates nanos1 3′UTR. Open box indicates miR-430 target site and gray box indicates miR-204 site. GFP reporter mRNA was injected at the one-cell stage, and GFP expression in PGCs was assayed at 26 hpf. To visualize PGCs, DsRed mRNA containing the nanos1 3′UTR was co-injected. GFP expression levels in PGCs and somatic cells are indicated on the right. N.D : not determined.(B–H) Expression of GFP reporter in gonad region (green) at 26 hpf. (B’–H’) Control DsRed expression in PGCs (red). Scale bar in H’shows 100μm.