Figure 6.
Correlation of enhancer activity with genomic features of preCRMs and distribution of segments by RP score and validation rates. (A) Correlation of enhancer activity with RP score. The negative log10 P-value for the difference in activity of each preCRM being different from that of preNeutrals is plotted against the mean RP score for the preCRM. The more significant activity from either the transient transfection or the site-directed stable integration assay was chosen for each preCRM. For stable transfection data, the smallest P-value during induction was used. (B) Correlation of enhancer activity with the number of ccGATA-1BSs in each preCRM. The values on the vertical axis are the same as in panel A, and the horizontal axis gives the number of matches to conserved consensus GATA-1 binding sites in each preCRM. (C) The distribution of DNA segments in the eight target loci after segmentation by RP score. Runs of at least 100 nucleotides of mouse DNA with mean RP scores within a designated range (bin in the histogram) were identified, and the number of segments in each bin of RP score is plotted. (Dark gray bars) Validation rate for DNA fragments containing at least one ccGATA1BS in each RP bin; the number of fragments tested in the enhancer assays is given on the top of each bin.