Figure 4.
Real-time RT-PCR analysis of selected genes in CCC or DCM myocardium. A: Chemokine/chemokine receptor genes. B: Genes identified by microarray analysis. C: Analysis of ANF expression in fetal cardiomyocytes after 8 hours of stimulus with MCP-1, IFN-γ and their combination. M, culture medium. Cardiomyocyte culture performed as described.17 In A and B, results are expressed as average fold change from normal heart (column) and SD (error bar). Asterisks, P < 0.05 versus normal. Human (A, B) or murine (C) GAPDH was used as the reference mRNA species, and GAPDH Ct values did not change significantly between disease categories or in vitro stimulus.