Figure 1.
Cluster analysis of gene expression (Cardiochip version 6.0 microarray data) in the CCC, DCM, and N left ventricular-free wall heart tissue mRNA samples. Data reveals CCC- and DCM-specific global gene expression profiles, with disease-specific and shared clusters of up- or down-regulated genes. Each individual column stands for the gene expression pattern of an individual heart mRNA sample belonging to CCC, DCM, or N groups; green dendrograms on the top of the figure represent degrees of similarity of gene expression among samples. Each horizontal line (y-axis) corresponds to the expression of a single gene across all samples. Gene expression clusters (green dendrograms to the left of the figure) represent relatedness of expression patterns among the 10,368 genes across several samples and patient groups. Expression values for each gene in each sample in relation to average control values normal tissue samples are expressed in a color scale (right of figure): yellow, unchanged; orange-red, up-regulated; blue, down-regulated. Nonsupervised hierarchical clustering performed with Genespring version.6.0, using correlation by up-regulated expression with default parameters, of microarray results for five CCC, seven DCM, and four normal heart tissue samples. Samples from each disease group are marked to facilitate their identification, with red, green, and white, standing for samples for CCC, DCM, and normal donor heart, respectively.