Figure 1.
pH dependency of the hydrolysis of 2 μM [3H]-AEA by rat brain homogenates. Two different buffer systems were used: (a) 100 mM sodium acetate buffer, 100 mM tris-HCl buffer, and 100 mM disodium tetraborate buffer (b) 50 mM sodium citrate, 50 mM tris-HCl and 50 mM sodium carbonate buffer. The pH values shown are the actual assay pH values, tested in scaled up assays, rather than the nominal pH of the buffers added to the homogenates. Data are means from two separate experiments using an incubation time of 20 min and of 4 μg protein/assay.