FIG. 6.
NL4-3 strains lacking the envelope gene retain their cytopathic effect. (A) Dot plot and histograms exhibiting the fraction of viable cells by FSC-SSC profiles (left panels) and the fraction of infected Jurkat 1.9 cells by HSA at days 1, 2, and 5 contained within the live gate for cultures mock infected, infected with NL4-3HSA env+ virus (E+), or infected with NL4-3HSA env− virus (E−) at an MOI of 10 (right panels). The numbers indicate the percentage of cells within the gates shown. (B) Quantitation of the fraction of viable cells (top panels) and the fraction of infected cells indicated by HSA (bottom panels) as a function of time for cultures of mock infected (⧫), NL4-3HSA env+ virus infected (▴), or NL4-3HSA env− virus infected (▪) in independent experiments with either Jurkat 1.9 or Jurkat 3 cells as indicated. Insets show the level of CD4 on the Jurkat sublines used. (C) Dot plot and histograms of mock and E− infections exhibiting a gate of nonviable cells by FSC-SSC profiles (left panels) that were then analyzed for the percentage of infected Jurkat 1.9 cells or Jurkat 3 cells by HSA at day 5. The numbers indicate the fraction of the cells within the gates shown. The results are representative of 20 experiments.