Figure 4.
Quantification of species in complex mixtures (‘Dilution Pools’). True relative abundance is compared with observed log (Cy5/Cy3) ratio for the 101 species with 1 or more species-specific probes. The x-axis corresponds to the six different relative abundance levels used in this experiment. The y-axis shows the distribution of observed log (Cy5/Cy3) ratios for species at the specified relative abundance level. When more than one probe was available for a given species, we averaged the log (Cy5/Cy3) ratios across all available probes (2–5 probes per species). Box-whisker plot format: box spans the 25% quantile to the 75% quantile surrounding the median; ‘whiskers,’ extend to span the full dataset excluding outliers; outliers are defined as points beyond 3/2 the interquantile range from the edge of the box.