FIG. 6.
(A) Growth of HeLa cells after transfection with hMOF or control siRNA. Error bars indicate standard errors of the means. (B) FACS profile of hMOF-depleted and control cells 4 days after transfection. The percentage of cells in each phase of the cell cycle is also indicated. (C) The PIKK inhibitor caffeine partly suppresses the accumulation of hMOF-depleted cells in G2/M. Caffeine-treated (2 mM, 24 h) or untreated HeLa cells were analyzed by FACS 4 days after transfection of hMOF siRNA or control siRNA. Bars indicate percentages of G2/M cells. Error bars indicate standard errors of the means. (E) hMOF-depleted cells show an increase in ATM S1981 phosphorylation. (E) Kinetics of DSB repair in hMOF-depleted and control cells after irradiation (IR) (1 Gy). At each time point, ATM pS1981 foci were counted from 50 randomly selected cells. Error bars indicate standard errors of the means within the experiment. Inset, example of ATM pS1981 and γH2AX foci 4 h after 1-Gy irradiation in control (top) and hMOF-depleted (bottom) cells.