FIG. 5.
p53 inhibition increases viral titers. Confluent cultures of parental A549 cells, i.e., A549 p53 dominant-negative clones 3 and 6 (A) or MEF p53−/− and MEF p53+/+ cells (B) were infected with PR8 at an MOI of 0.05. At various times postinfection, supernatants were collected and viral titers determined by TCID50 on MDCK cells. (C) Confluent cultures of parental A549 cells and p53 dominant-negative clones 3 and 6 were infected with PR8 at an MOI of 2. At various times postinfection, supernatants were collected and viral titers determined by use of the TCID50 for MDCK cells. Asterisks indicate samples that are significant (P < 0.05) as determined by ANOVA.