Skip to main content
NCBI home page
Biochemical Journal logoLink to Biochemical Journal
. 1981 Sep 15;198(3):691–698. doi: 10.1042/bj1980691

The role of potassium transport in the generation of a pH gradient in Escherichia coli.

R G Kroll, I R Booth
PMCID: PMC1163319  PMID: 7034732

Abstract

The role of K+ transport in the generation of a pH gradient in Escherichia coli has been investigated. In K+-depleted cells, net K+ uptake dissipated delta psi (membrane potential) and led to an increase in delta pH (pH gradient). The magnitude of the delta pH formed bore a simple relationship to the net K+ uptake and was substantially independent of the respiratory rate. In K+-replete cells, generation of a pH gradient was again K+-dependent, although no net uptake of this cation occurred. The results are discussed in terms of K+ cycling, and it is suggested that delta pH is in part a function of the rate of cycling and independent of the respiratory rate.

Full text

PDF
691

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Booth I. R., Mitchell W. J., Hamilton W. A. Quantitative analysis of proton-linked transport systems. The lactose permease of Escherichia coli. Biochem J. 1979 Sep 15;182(3):687–696. doi: 10.1042/bj1820687. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brey R. N., Rosen B. P., Sorensen E. N. Cation/proton antiport systems in Escherichia coli. Properties of the potassium/proton antiporter. J Biol Chem. 1980 Jan 10;255(1):39–44. [PubMed] [Google Scholar]
  3. Epstein W., Kim B. S. Potassium transport loci in Escherichia coli K-12. J Bacteriol. 1971 Nov;108(2):639–644. doi: 10.1128/jb.108.2.639-644.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Epstein W., Schultz S. G. Cation Transport in Escherichia coli: V. Regulation of cation content. J Gen Physiol. 1965 Nov 1;49(2):221–234. doi: 10.1085/jgp.49.2.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harold F. M. Ion currents and physiological functions in microorganisms. Annu Rev Microbiol. 1977;31:181–203. doi: 10.1146/annurev.mi.31.100177.001145. [DOI] [PubMed] [Google Scholar]
  6. Kell D. B. On the functional proton current pathway of electron transport phosphorylation. An electrodic view. Biochim Biophys Acta. 1979 Jul 3;549(1):55–99. doi: 10.1016/0304-4173(79)90018-1. [DOI] [PubMed] [Google Scholar]
  7. Krulwich T. A., Mandel K. G., Bornstein R. F., Guffanti A. A. A non-alkalophilic mutant of Bacillus alcalophilus lacks the Na+/H+ antiporter. Biochem Biophys Res Commun. 1979 Nov 14;91(1):58–62. doi: 10.1016/0006-291x(79)90582-5. [DOI] [PubMed] [Google Scholar]
  8. Mitchell P. Chemiosmotic coupling in oxidative and photosynthetic phosphorylation. Biol Rev Camb Philos Soc. 1966 Aug;41(3):445–502. doi: 10.1111/j.1469-185x.1966.tb01501.x. [DOI] [PubMed] [Google Scholar]
  9. Mitchell P. Performance and conservation of osmotic work by proton-coupled solute porter systems. J Bioenerg. 1973 Jan;4(1):63–91. doi: 10.1007/BF01516051. [DOI] [PubMed] [Google Scholar]
  10. Padan E., Zilberstein D., Rottenberg H. The proton electrochemical gradient in Escherichia coli cells. Eur J Biochem. 1976 Apr 1;63(2):533–541. doi: 10.1111/j.1432-1033.1976.tb10257.x. [DOI] [PubMed] [Google Scholar]
  11. Plack R. H., Jr, Rosen B. P. Cation/proton antiport systems in Escherichia coli. Absence of potassium/proton antiporter activity in a pH-sensitive mutant. J Biol Chem. 1980 May 10;255(9):3824–3825. [PubMed] [Google Scholar]
  12. Reider E., Wagner E. F., Schweiger M. Control of phosphoenolpyruvate-dependent phosphotransferase-mediated sugar transport in Escherichia coli by energization of the cell membrane. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5529–5533. doi: 10.1073/pnas.76.11.5529. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhoads D. B., Epstein W. Cation transport in Escherichia coli. IX. Regulation of K transport. J Gen Physiol. 1978 Sep;72(3):283–295. doi: 10.1085/jgp.72.3.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rhoads D. B., Waters F. B., Epstein W. Cation transport in Escherichia coli. VIII. Potassium transport mutants. J Gen Physiol. 1976 Mar;67(3):325–341. doi: 10.1085/jgp.67.3.325. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schuldiner S., Fishkes H. Sodium-proton antiport in isolated membrane vesicles of Escherichia coli. Biochemistry. 1978 Feb 21;17(4):706–711. doi: 10.1021/bi00597a023. [DOI] [PubMed] [Google Scholar]
  16. Stock J. B., Rauch B., Roseman S. Periplasmic space in Salmonella typhimurium and Escherichia coli. J Biol Chem. 1977 Nov 10;252(21):7850–7861. [PubMed] [Google Scholar]
  17. Yamasaki K., Moriyama Y., Futai M., Tsuchiya T. Uptake and extrusion of k+ regulated by extracellular pH in Escherichia coli. FEBS Lett. 1980 Oct 20;120(1):125–127. doi: 10.1016/0014-5793(80)81061-1. [DOI] [PubMed] [Google Scholar]
  18. Zilberstein D., Padan E., Schuldiner S. A single locus in Escherichia coli governs growth in alkaline pH and on carbon sources whose transport is sodium dependent. FEBS Lett. 1980 Jul 28;116(2):177–180. doi: 10.1016/0014-5793(80)80637-5. [DOI] [PubMed] [Google Scholar]
  19. Zilberstein D., Schuldiner S., Padan E. Proton electrochemical gradient in Escherichia coli cells and its relation to active transport of lactose. Biochemistry. 1979 Feb 20;18(4):669–673. doi: 10.1021/bi00571a018. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES

OSZAR »