FIG. 2.
Activation of NF-κB by delNS1 virus infection in MEFs. (A) Detection of activated NF-κB by EMSA. MEFs were untreated (UT), mock infected, or infected with PR8, delNS1, or NDV viruses at an MOI of 1 for the indicated times. Nuclear extracts were subjected to EMSA with a DNA probe specific for NF-κB. TNF-α- and dsRNA-treated cells were included as positive controls. (B) Supershift of NF-κB complexes. Anti-p65 and anti-p50 antibodies (Ab) were used to shift the NF-κB complexes in delNS1- or NDV-infected MEFs at 6 h p.i.