Fig. 5. Germline-specific and enriched sequences and genes in hagfish.
a, Plot showing the degree of germline enrichment and estimated span of all predicted repetitive elements in the E. atami genome, focusing on elements with a cumulative span of less than 4 Mb (per family member). Previously identified elements30,33 are highlighted by coloured circles and newly identified high-copy elements are highlighted by coloured diamonds. Additional higher copy repeats are visible in Extended Data Fig. 12m,n. The colouring scheme is the same in b and in Extended Data Fig. 12m,n. b, Estimated cumulative span of the eight most highly abundant repeats shown as the percentage of the genome covered. c, Fluorescence in situ hybridization (FISH) of high-copy germline-specific repeats to a testes metaphase plate showing their distinct spatial clustering within chromosomes (blue counterstaining is NucBlue: Hoechst 33342; individual pairs of probes are shown in Extended Data Fig. 12m,n). d, Comparison of the sequence depth of DNA extracted from germline (testes) versus somatic (blood) tissues identifies a large number of genomic intervals with evidence for strong enrichment in the germline. The bin representing no enrichment contains a total of 2.3 Gb of the assembly. e, Genes encoded within germline-specific regions are enriched for several ontology terms related to regulation of cell cycle and cell motility (Panther Biological Processes: most specific subclass shown; Supplementary Table 14).