Extended Data Fig. 10. Gene expression and gene duplications in vertebrates.
a,b, Weighted gene co-expression network analysis (WGCNA) among organs for hagfish (a) and gar (b). Each row corresponds to a WGCNA cluster (with an arbitrary colour name) and its expression specificity is shown in selected tissues on the left (a, hagfish, b, gar). The enrichment of gene duplicated at successive phylogenetic nodes in each WGCNA cluster is indicated on the right as the p-value (-log10) of hypergeometric tests. A significant enrichment is observed in gene with strong neural expression (brain, blue cluster). c, Expression of selected paralogues involved in neural crest specification and migration in cranial and trunk neural crest tissues from lamprey P. marinus. RNA-seq data from a previous study56 was quantified using the latest version of the lamprey genome and RefSeq annotation (kPetMar1). For each gene family, all paralogues derived from the vertebrate polyploidization event (1RV and 2RCY) are considered and classified (see Supplementary Tables 9 and 10). As denoted in inset, 1 (green cells) and 2 (pink cells) refer to the two original paralog branches derived from 1RV (see main Fig. 4a). Grey groups could not be definitively assigned.