FIG. 2.
Surface localization of the 72- and 85-kDa proteins from SK-SY-5Y and U937 cells. Surface biotinylated proteins from SK-SY-5Y (A and C) and U937 cells (B) were passed through the column with (A and B) or without (C) recombinant E protein coupled to NiNTA-agarose. Ten microliters of total biotinylated proteins (lanes 1) and 50 μl of the elution fraction (lanes 2) were separated by SDS-10% PAGE, transferred to nitrocellulose membrane, and revealed with streptavidin-peroxidase. Molecular mass markers are indicated on the left. Arrows indicate migration of eluted 72- and 85-kDa proteins as well as a 60-kDa protein present only in U937 cells.