Table 3.
Activities of the redox enzymes catalase, Gpx and SOD, in differentiated NO resistant RAW 264 7 cells
| catalase (U/mg) | Gpx (U/mg) | SOD (U/mg) | |||||||
| time (hours) | 6 | 24 | 48 | 6 | 24 | 48 | 6 | 24 | 48 |
| control | 873+/-100 | 761+/-128 | 767+/-96 | 257+/-116 | 379+/-95 | 300+/-32 | 249+/-111 | 263+/-47 | 326+/-76 |
| LPS5 | 1563+/-408 | 1036+/-670 | 738+/-90 | 335+/-206 | 249+/-55 | 323+/-52 | 454+/-174 | 205+/-136 | 231+/-169 |
| IFN50 | 598+/81 | 555+/ 92 | 612+/76 | 420+/ 30 | 218+/46 | 248+/17 | 1637+/512 | 288+/ 85 | 66+/37 |
| LPS5/IFN50 | 778+/67 | 698+/167 | 849+/ 33 | 280+/13 | 213+/61 | 268+/ 18 | 1833+/1601 | 284+/ 190 | 236+/115 |
RAW 264.7 cells were cultured (10 × 106 cells in 50 ml) with medium or medium supplemented with either IFN-γ (50 U/ml), or LPS (5 μg/ml) or a mixture of IFN-γ (50 U/ml) + LPS (5 μg/ml). Cells were harvested at 6, 24, and 48 hours. Activities of the enzymes : catalase (decomposition of H2O2), Gpx (oxidation of NADPH) and SOD (reaction with chromophore) were determined on cell lysates as described in Methods. Results are expressed as means +/- SEM of five independent experiments.