Table 1. Characterization of OsPolI-like DNA polymerase domain: template/primer specificity.
Template/primer | KCl concentration (mM) | Activity (%) |
---|---|---|
Poly(dA)/oligo(dT)12–18 | 0 | 100 |
100 | 66.6 | |
200 | 57.3 | |
Poly(rA)/oligo(dT)12–18 | 0 | 11.2 |
100 | 4.9 | |
200 | 2.0 | |
Poly(dA-dT) | 0 | 26.1 |
100 | 13.5 | |
200 | 3.0 | |
Activated DNA | 0 | 40.0 |
100 | 18.7 | |
200 | 5.1 |
DNA polymerase activity was measured as described in Materials and Methods. The activity with poly(dA)/oligo(dT) in the absence of KCl was expressed as 100%.