FIG. 1.
Generation of XIAP-deficient mice by gene targeting. (A) Schematic representation of the targeting strategy used to disrupt the XIAP gene. The locations of exons 1 and 6 are shown. Exons 2 to 5 are contained within the 8.3-kb HindIII-HindIII fragment (not shown). The targeting construct replaces the coding sequence of exon 1 with the neomycin resistance gene, in the opposite transcriptional orientation. The sequence used as a probe to screen for homologously recombined DNA is shown. B (BglII) and H (HindIII) sites are shown for the genomic and targeted DNA. (B) Southern blot analysis of BglII-digested tail DNA from targeted mice. The WT allele is 4.0 kb, and the targeted allele is 3.2 kb. The blots were probed with the 0.2-kb BamHI-EcoRI fragment shown in panel A.